Experimental Protocols in Biotechnology
portes grátis
Experimental Protocols in Biotechnology
;
Springer-Verlag New York Inc.
06/2020
242
Dura
Inglês
9781071606063
Pré-lançamento - envio 15 a 20 dias após a sua edição
Descrição não disponível.
1. Microbial TechniquesShilpa Deshpande Kaistha, Vineet Kumar, Pramila Umrao Department of Microbiology, Institute of Biosciences &Biotechnology, CSJMU, Kanpur 1.1. Introduction 1.2. Technique 1.2.1. Preparation of culture media and its sterilization 1.2.2. Microbial Plating Techniques 1.2.3. Streak plate method 1.2.4. Antibiotic Sensitivity Test (AST) 1.2.5. Kirby Bauer Disk diffusion method 1.2.6. Rapid Minimum Inhibitory Concentration determination using E-test 1.3. Microbiology Quality Control testing of Dairy Products 1.3.1. Standard Plate Count for Bacteria, Yeasts and Molds 1.3.2. Breed Count 1.3.3. Methylene Blue Reduction Test 1.4. Serological tests for Microbial Detection 1.4.1. Agglutination 1.4.2. Enzyme Linked Immunosorbent Assay (ELISA) 1.5. References 2. Protozoa detection and Isolation Neelima Gupta Vice Chancellor, CSJMU, Kanpur 2.1. Introduction 2.2. Technique 2.2.1. Detection of Protozoa from blood samples 2.2.2. Isolation and processing of Protozoan parasites 2.3. References 3. Nematodes - Isolation, Detection and Molecular characterization Aashaq Hussain Bhat, Anshika* , Ashok Kumar Chaubey and Sandeep Kumar Malhotra* Nematology Laboratory, Department of Zoology,Ch. Charan Singh University, Meerut-250004 *Parasitology Laboratory, Department of Zoology, University of Allahabad, Allahabad-211 002, U.P., India 3.1. Introduction 3.2. Technique 3.3. Materials and Methods 3.3.1. Animal Parasitic worms 3.3.2. Plant Parasitic worms 3.3.3. Extraction of Free Living and Ectoparasites 3.4. Killing and Fixation 3.5.Preparation of Nematode Mounts 3.6. Preservation of Nematodes 3.7.Molecular investigations 3.7.1. Genomic DNA Extraction 3.7.2. Agarose Gel Electrophoresis 3.7.3. PCR Amplification 3.7.4. DNA Barcoding 3.8. References 4. Insects Collection, Preservation, Identification and Characterization Kailash Chandra, Vikas Kumar, Kaomud Tyagi, Sandeep Kushwaha, Shantanu Kundu Centre for DNA Taxonomy, Molecular Systematics Division, Zoological Survey of India, M Block, New Alipore, Kolkata-53. 4.1. Introduction 4.2. Technique 4.3. Materials and Methods 4.3.1. Collection of Insects 4.3.2. Killing of Insects 4.3.3. Identification of Insects 4.3.4. DNA Extraction from Insects 4.3.5. DNA Quantification and Agarose Gel Electrophoresis 4.3.6. Polymerase Chain Reaction 4.3.7. DNA Sequencing 4.4. In-Silico Analysis 4.5. Formatting of sequences 4.5.1. Sequence Similarity Search 4.5.2. Sequence Annotation and Submission 4.5.3. Phylogenetic analysis based on DNA barcode 4.6.References 5. Acid Phosphatase assay from Plant source (Section A) Annika Singh Department of Biotechnology, Chhatrapati Shahu Ji Maharaj University, Kanpur 5.1A. Introduction 5.2A. Technique 5.2.1A. Standard Curve 5.2.2A. Phosphatase Kinetics 5.3A. References Assays of Superoxide dismutase in clinical samples by auto-oxidation of epinephrine (Section B) Dinesh Kumar, Payal Singh Raghuvanshi, Sipahee Lal Patel, Anamika Dwivedi and Varsha Gupta Rheumatology Laboratory, Department of Biotechnology, CSJMU, Kanpur 5.1B. Introduction 5.2B. Technique 5.3B. Materials and methods 5.3.1B. Preparation of standard curve 5.3.2B. Calculation of molar extinction coefficient from absorbance 5.3.3B. Determination of initial velocity (Vo) 5.3.4B. Determination of substrate and product concentration 5.3.5B. Calculating Enzyme Activity 5.3.6B. Determination of Km and Vmax Values for Michaelis Menten plot 5.3.7B. Graphically representation Lineweaver-Burke, Hen's, Eadie- Hofstee plot 5.4B. References 6. Detection of phospho-proteins (phospho-serine and phospho-threonine) from Plant system using Western Blotting Varsha Gupta1 and Baishnab Charan Tripathy2 1Rheumatology Laboratory, Department of Biotechnology, CSJMU, Kanpur; 2School of Life sciences, Jawaharlal Nehru University, New Delhi 6.1. Introduction 6.2.Technique 6.3. Materials and methods 6.3.1. Collection of tissue 6.3.2. Isolation of Thylakoid membranes 6.3.3. Protein estimation 6.3.4. Sodiumdodecyl polyacryamide gel electrophoresis (SDS-PAGE) 6.3.5. Western blotting 6.3.6. Detection of phospho-proteins using antibody 6.4. References 7. Gene Expression Analysis from human blood peripheral blood mononuclear cells Sipahee Lal Patel, Dinesh Kumar, Anamika Dwivedi, Payal Singh Raghuvanshi, Vishal Chand, and Varsha Gupta Rheumatology Laboratory, Department of Biotechnology, CSJMU, Kanpur 7.1.Introduction 7.2.Technique 7.3.Materials and Methods 7.3.1. Total RNA isolation from human blood 7.3.2. RNA quantification and quality check by Agarose Gel Electrophoresis 7.3.3. cDNA synthesis using poly-dT primers with Reverse Transcriptase 7.3.4. Real-time Polymerase chain reaction using SYBR green dye (Q-PCR) 7.4.References 8. Bioinformatics tools Dev Bukhsh Singh Department of Biotechnology, CSJMU, Kanpur 8.1. Introduction 8.2. Technique 8.3.Materials and Methods 8.3.1. Modeling the 3D structure of a protein using homology 8.3.2. Computational designing of ligands and understanding Molecular Interaction 8.3.2. Systems biology approach for Modeling the structure and dynamics of Biological Pathways 8.4. References 9. Appendix (Buffers composition) 10. Trouble shooting 11. Index
Este título pertence ao(s) assunto(s) indicados(s). Para ver outros títulos clique no assunto desejado.
1. Microbial TechniquesShilpa Deshpande Kaistha, Vineet Kumar, Pramila Umrao Department of Microbiology, Institute of Biosciences &Biotechnology, CSJMU, Kanpur 1.1. Introduction 1.2. Technique 1.2.1. Preparation of culture media and its sterilization 1.2.2. Microbial Plating Techniques 1.2.3. Streak plate method 1.2.4. Antibiotic Sensitivity Test (AST) 1.2.5. Kirby Bauer Disk diffusion method 1.2.6. Rapid Minimum Inhibitory Concentration determination using E-test 1.3. Microbiology Quality Control testing of Dairy Products 1.3.1. Standard Plate Count for Bacteria, Yeasts and Molds 1.3.2. Breed Count 1.3.3. Methylene Blue Reduction Test 1.4. Serological tests for Microbial Detection 1.4.1. Agglutination 1.4.2. Enzyme Linked Immunosorbent Assay (ELISA) 1.5. References 2. Protozoa detection and Isolation Neelima Gupta Vice Chancellor, CSJMU, Kanpur 2.1. Introduction 2.2. Technique 2.2.1. Detection of Protozoa from blood samples 2.2.2. Isolation and processing of Protozoan parasites 2.3. References 3. Nematodes - Isolation, Detection and Molecular characterization Aashaq Hussain Bhat, Anshika* , Ashok Kumar Chaubey and Sandeep Kumar Malhotra* Nematology Laboratory, Department of Zoology,Ch. Charan Singh University, Meerut-250004 *Parasitology Laboratory, Department of Zoology, University of Allahabad, Allahabad-211 002, U.P., India 3.1. Introduction 3.2. Technique 3.3. Materials and Methods 3.3.1. Animal Parasitic worms 3.3.2. Plant Parasitic worms 3.3.3. Extraction of Free Living and Ectoparasites 3.4. Killing and Fixation 3.5.Preparation of Nematode Mounts 3.6. Preservation of Nematodes 3.7.Molecular investigations 3.7.1. Genomic DNA Extraction 3.7.2. Agarose Gel Electrophoresis 3.7.3. PCR Amplification 3.7.4. DNA Barcoding 3.8. References 4. Insects Collection, Preservation, Identification and Characterization Kailash Chandra, Vikas Kumar, Kaomud Tyagi, Sandeep Kushwaha, Shantanu Kundu Centre for DNA Taxonomy, Molecular Systematics Division, Zoological Survey of India, M Block, New Alipore, Kolkata-53. 4.1. Introduction 4.2. Technique 4.3. Materials and Methods 4.3.1. Collection of Insects 4.3.2. Killing of Insects 4.3.3. Identification of Insects 4.3.4. DNA Extraction from Insects 4.3.5. DNA Quantification and Agarose Gel Electrophoresis 4.3.6. Polymerase Chain Reaction 4.3.7. DNA Sequencing 4.4. In-Silico Analysis 4.5. Formatting of sequences 4.5.1. Sequence Similarity Search 4.5.2. Sequence Annotation and Submission 4.5.3. Phylogenetic analysis based on DNA barcode 4.6.References 5. Acid Phosphatase assay from Plant source (Section A) Annika Singh Department of Biotechnology, Chhatrapati Shahu Ji Maharaj University, Kanpur 5.1A. Introduction 5.2A. Technique 5.2.1A. Standard Curve 5.2.2A. Phosphatase Kinetics 5.3A. References Assays of Superoxide dismutase in clinical samples by auto-oxidation of epinephrine (Section B) Dinesh Kumar, Payal Singh Raghuvanshi, Sipahee Lal Patel, Anamika Dwivedi and Varsha Gupta Rheumatology Laboratory, Department of Biotechnology, CSJMU, Kanpur 5.1B. Introduction 5.2B. Technique 5.3B. Materials and methods 5.3.1B. Preparation of standard curve 5.3.2B. Calculation of molar extinction coefficient from absorbance 5.3.3B. Determination of initial velocity (Vo) 5.3.4B. Determination of substrate and product concentration 5.3.5B. Calculating Enzyme Activity 5.3.6B. Determination of Km and Vmax Values for Michaelis Menten plot 5.3.7B. Graphically representation Lineweaver-Burke, Hen's, Eadie- Hofstee plot 5.4B. References 6. Detection of phospho-proteins (phospho-serine and phospho-threonine) from Plant system using Western Blotting Varsha Gupta1 and Baishnab Charan Tripathy2 1Rheumatology Laboratory, Department of Biotechnology, CSJMU, Kanpur; 2School of Life sciences, Jawaharlal Nehru University, New Delhi 6.1. Introduction 6.2.Technique 6.3. Materials and methods 6.3.1. Collection of tissue 6.3.2. Isolation of Thylakoid membranes 6.3.3. Protein estimation 6.3.4. Sodiumdodecyl polyacryamide gel electrophoresis (SDS-PAGE) 6.3.5. Western blotting 6.3.6. Detection of phospho-proteins using antibody 6.4. References 7. Gene Expression Analysis from human blood peripheral blood mononuclear cells Sipahee Lal Patel, Dinesh Kumar, Anamika Dwivedi, Payal Singh Raghuvanshi, Vishal Chand, and Varsha Gupta Rheumatology Laboratory, Department of Biotechnology, CSJMU, Kanpur 7.1.Introduction 7.2.Technique 7.3.Materials and Methods 7.3.1. Total RNA isolation from human blood 7.3.2. RNA quantification and quality check by Agarose Gel Electrophoresis 7.3.3. cDNA synthesis using poly-dT primers with Reverse Transcriptase 7.3.4. Real-time Polymerase chain reaction using SYBR green dye (Q-PCR) 7.4.References 8. Bioinformatics tools Dev Bukhsh Singh Department of Biotechnology, CSJMU, Kanpur 8.1. Introduction 8.2. Technique 8.3.Materials and Methods 8.3.1. Modeling the 3D structure of a protein using homology 8.3.2. Computational designing of ligands and understanding Molecular Interaction 8.3.2. Systems biology approach for Modeling the structure and dynamics of Biological Pathways 8.4. References 9. Appendix (Buffers composition) 10. Trouble shooting 11. Index
Este título pertence ao(s) assunto(s) indicados(s). Para ver outros títulos clique no assunto desejado.
